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Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Prematurity reduces citrulline-arginine-nitric oxide production and precedes the onset of necrotizing enterocolitis in piglets
doi: 10.1152/ajpgi.00198.2018
Figure Lengend Snippet: Multiple end-point measurements of necrotizing enterocolitis (NEC) incidence and severity in piglets. A: Kaplan-Meir graph detailing piglet survival. B: gross NEC score in piglets of advancing gestational age exposed to the NEC protocol. A threshold of 3 in any one segment was considered positive for NEC (dashed line). C: histological NEC score was determined in hematoxylin-eosin-stained tissue sections, and a threshold of ≥2 in any tissue section was considered positive for NEC (dashed line). D: plasma intestinal fatty-acid binding protein (iFABP) concentration in the final plasma sample for piglet groups in response to the NEC protocol. B–D: *P < 0.05, significant differences between groups, and error bars show 95% confidence interval. Data analyzed by Kruskal-Wallis test and Dunn’s multiple comparison test. NS, not significant.
Article Snippet: Amino acid concentrations in plasma and feed were measured using phenyl isothiocyanate derivatives relative to a methionine sulfone internal standard by HPLC ( 6 ). iFABP was measured using a
Techniques: Staining, Clinical Proteomics, Binding Assay, Concentration Assay, Comparison